MY ROUTINE OF SEMEN ANALYSIS The purpose of my routine of semen analysis is to give all the informations needful for the diagnosis. It evaluates several seminal parameters, in order to obtain data of clinical significance to investigate the functional activity of the male genital organs, as follows: TO EVALUATE THE TESTICULAR AND EPIDIDYMAL FUNCTION Sperm concentration Sperm morphology including strict criteria Acrosome status Nuclear integrity Plasma membrane integrity Immature germ cell concentration Differential analysis of immature germ cells Sertoli cells Count Alkaline phosphatase activity Analysis of sperm motility Analysis of sperm necrosis TO EVALUATE THE SECRETORY ACTIVITY OF THE SEMINAL VESICLES Fructose Inorganic phosphorus Physical examination (coagulation, liquefaction, volume, viscosity and pH) Count of seminal vesicles epithelial cells TO EVALUTE THE SECRETORY ACTIVITY OF THE PROSTATE Total calcium Acid phosphatase activity Zinc Physical examination (coagulation, liquefaction, volume, viscosity and pH) Count of prostate epithelial cells OTHER TESTS Auto-mmunity (sperm agglutination, pseudoaglutination, immobilization) Antisperm antibodies (MAR-test) Bacterioscopic examination Leukocyte count Differential leukocyte count Seminophages count Differential seminophages count Red Blood cells count Residual bodies count Sperm recovery (swin-up) Reactive oxygen species in leukocytes and spermatozoa Hypoosmotic swelling test