- - User Protein Accession Number RTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK Peptide N-terminal Gln to pyroGlu Oxidation of M Protein N-terminus Acetylated - Trypsin 1 AND Hydrogen Free Acid C2 H3 N1 O1 carbamidomethylation MALDI-TOF 0 0 0 800 4000 5 MALDI-TOF 0 0 - 5.5 47200 A64 C4 D28 E24 F8 G45 H10 I16 K28 L39 M8 N20 P21 Q22 R14 S22 T40 V23 W3 Y11 806.4776 806.9868 353 359 1 R ALEFAKK E 888.4249 889.0246 179 186 0 K CPGNALEK G 888.4791 889.0026 201 210 0 R ADVTLGGGAK T 960.5002 961.0665 36 44 0 R DSLSDKPAK N 1045.5642 1046.1759 26 35 0 R LTGDQTAALR D 1072.5863 1073.2046 225 233 1 K TLREQAQAR G 1099.4730 1100.1999 169 178 0 K CYGPSATSEK C 1113.6268 1114.2952 384 394 0 K APGLTQALNTK D 1130.5628 1131.3042 179 189 1 K CPGNALEKGGK G 1144.6578 1145.3499 307 316 1 K AIELLSKNEK G 1154.5880 1155.3671 129 138 0 K DHPTILEMAK A 1160.5376 1161.2674 64 74 0 R NYAEGAGGFFK G 1170.5829 1171.3665 1Met-ox 129 138 0 K DHPTILEMAK A 1184.6024 1185.2902 12 24 0 R AAQGDITAPGGAR R 1185.5938 1186.3812 2 11 0 R TPEMPVLENR A 1201.5887 1202.3805 1Met-ox 2 11 0 R TPEMPVLENR A 1201.6541 1202.3615 190 200 0 K GSITEQLLNAR A 1201.6653 1202.3645 25 35 1 R RLTGDQTAALR D 1227.5680 1228.3751 168 178 1 R KCYGPSATSEK C 1340.7035 1341.4788 12 25 1 R AAQGDITAPGGARR L 1341.6949 1342.5698 1 11 1 - RTPEMPVLENR A 1357.6898 1358.5692 1Met-ox 1 11 1 - RTPEMPVLENR A 1410.7269 1411.6079 317 329 0 K GFFLQVEGASIDK Q 1416.7123 1417.5714 116 128 0 K TYNGALGVDIHEK D 1419.6790 1420.5914 294 306 0 R NDSVPTLAQMTDK A 1435.6739 1436.5907 1Met-ox 294 306 0 R NDSVPTLAQMTDK A 1443.7920 1444.6412 187 200 1 K GGKGSITEQLLNAR A 1496.7021 1497.6146 211 224 0 K TFAETATAGEWQGK T 1781.9074 1783.0037 314 329 1 K NEKGFFLQVEGASIDK Q 1866.9350 1868.0694 211 227 1 K TFAETATAGEWQGKTLR E 1959.0221 1960.2713 45 63 0 K NIILLIGDGMGDSEITAAR N 1968.8795 1970.2012 169 186 1 K CYGPSATSEKCPGNALEK G 1975.0170 1976.2706 1Met-ox 45 63 0 K NIILLIGDGMGDSEITAAR N 1975.0289 1976.2540 75 92 0 K GIDALPLTGQYTHYALNK K 1987.0460 1988.2191 26 44 1 R LTGDQTAALRDSLSDKPAK N 2071.1148 2072.3408 190 210 1 K GSITEQLLNARADVTLGGGAK T 2103.1239 2104.4292 75 93 1 K GIDALPLTGQYTHYALNKK T 2174.1379 2175.5221 294 313 1 R NDSVPTLAQMTDKAIELLSK N 2190.1328 2191.5215 1Met-ox 294 313 1 R NDSVPTLAQMTDKAIELLSK N 2213.0726 2214.4088 94 115 0 K TGKPDYVTDSAASATAWSTGVK T 2240.0882 2241.5062 274 293 0 K ATYHGNIDKPAVTCTPNPQR N 2341.1676 2342.5840 93 115 1 K KTGKPDYVTDSAASATAWSTGVK T 2351.1778 2352.6481 2 24 1 R TPEMPVLENRAAQGDITAPGGAR R 2366.1628 2367.5938 201 224 1 R ADVTLGGGAKTFAETATAGEWQGK T 2367.1727 2368.6474 1Met-ox 2 24 1 R TPEMPVLENRAAQGDITAPGGAR R 2487.2843 2488.7775 360 383 0 K EGNTLVIVTADHAHASQIVAPDTK A 2506.1269 2507.6715 1pyroGlu 330 352 0 K QDHAANPCGQIGETVDLDEAVQR A 2523.1534 2524.7022 330 352 0 K QDHAANPCGQIGETVDLDEAVQR A 2552.2819 2553.9152 116 138 1 K TYNGALGVDIHEKDHPTILEMAK A 2568.2768 2569.9146 1Met-ox 116 138 1 K TYNGALGVDIHEKDHPTILEMAK A 2615.3793 2616.9526 359 383 1 K KEGNTLVIVTADHAHASQIVAPDTK A 2696.3394 2698.0895 420 444 0 R IAAYGPHAANVVGLTDQTDLFYTMK A 2712.3343 2714.0888 1Met-ox 420 444 0 R IAAYGPHAANVVGLTDQTDLFYTMK A 2727.1626 2728.9050 395 419 0 K DGAVMVMSYGNSEEDSQEHTGSQLR I 2743.1576 2744.9043 1Met-ox 395 419 0 K DGAVMVMSYGNSEEDSQEHTGSQLR I 2759.1525 2760.9037 2Met-ox 395 419 0 K DGAVMVMSYGNSEEDSQEHTGSQLR I 2792.4543 2794.1136 139 167 0 K AAGLATGNVSTAELQDATPAALVAHVTSR K 2821.4208 2823.2263 269 293 1 R WLGPKATYHGNIDKPAVTCTPNPQR N 2900.5039 2902.3145 36 63 1 R DSLSDKPAKNIILLIGDGMGDSEITAAR N 2916.4988 2918.3139 1Met-ox 36 63 1 R DSLSDKPAKNIILLIGDGMGDSEITAAR N 2920.5492 2922.2888 139 168 1 K AAGLATGNVSTAELQDATPAALVAHVTSRK C 3100.5414 3102.5154 45 74 1 K NIILLIGDGMGDSEITAARNYAEGAGGFFK G 3116.5363 3118.5147 1Met-ox 45 74 1 K NIILLIGDGMGDSEITAARNYAEGAGGFFK G 3116.5482 3118.4981 64 92 1 R NYAEGAGGFFKGIDALPLTGQYTHYALNK K 3165.4911 3167.4598 1pyroGlu 330 358 1 K QDHAANPCGQIGETVDLDEAVQRALEFAK K 3182.5177 3184.4905 330 358 1 K QDHAANPCGQIGETVDLDEAVQRALEFAK K 3249.6982 3251.7964 420 450 1 R IAAYGPHAANVVGLTDQTDLFYTMKAALGLK - 3265.6931 3267.7958 1Met-ox 420 450 1 R IAAYGPHAANVVGLTDQTDLFYTMKAALGLK - 3581.8927 3584.0494 360 394 1 K EGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTK D 3610.7665 3612.9569 94 128 1 K TGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEK D 3640.7488 3643.0743 274 306 1 K ATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDK A 3656.7437 3659.0737 1Met-ox 274 306 1 K ATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDK A 3703.8754 3706.1957 234 268 0 R GYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVR W 3719.8703 3722.1951 1Met-ox 234 268 0 R GYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVR W 3821.7711 3824.1769 384 419 1 K APGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLR I 3837.7660 3840.1762 1Met-ox 384 419 1 K APGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLR I 3853.7609 3856.1756 2Met-ox 384 419 1 K APGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLR I 3914.8619 3917.2867 317 352 1 K GFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQR A 3928.0238 3930.4574 129 167 1 K DHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSR K 3944.0188 3946.4567 1Met-ox 129 167 1 K DHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSR K