Table-2: Analysis of LD1 suppression by SUC1 (modified from Bennoun and Delosme, 1999) [scale of *(0-4): (*) = slow, (****) = fast, wild type growth]
|
Cross
1)LD1 MUD2 mt- (*) x WT mt+ (****)
2)LD1 MUD2 SUC1 mt- (***) x WT mt+ (****)
3) WT mt- (*) x LD1 MUD2 SUC1 mt+ (***)
4) LD MUD2 mt- (*) x LD1 MUD2 SUC1 mt+ (***)
5) LD1 MUD2 SMr mt- (*) x SUC1 mt+ (***)
6) LD1 MUD2 SMr mt- (*) x SUC1 SMs mt+ (***)
|
# of crosses
8
4
2
2
9
1
|
Total No. of Tetrads
45
24
12
7
40
8
|
Number/Phenotype of Uniparental Progeny
45/(*) (strictly uniparental-paternal)
18/(*) (not strictly uniparental inheritance for SUC1)
12/(****) progeny were all fast growers
5/(***)
36/(***)
8/(***) fast growers and SMs
|
Back to chloroplast genetics essay.
Table-3: Analysis of LD1 suppression by SUC2 (modified from Bennoun and Delosme, 1999)
|
Cross
1) ac10-6C mt- (0) x ac10-6C SUC2 mt+ (***)
2) ac10-6C SUC2 mt- (***) x ac10-6C mt+ (0)
3) LD1 MUD2 mt- (*) x ac10-6C SUC2 mt+ (***)
4) LD1 MUD2 mt- (*) x WT mt+ (****)
5) WT mt- (****) x LD1 MUD2 ac10-6C SUC2 mt+ (***)
6) LD1 MUD2 mt- (*) x LD1 MUD2 ac10-6C SUC2 mt+ (***)
7) LD1 MUD2 SMr mt- (*) x LD1 MUD2 ac10-6C SUC2 mt+ (***)
|
# of crosses
2
1
5
3
1
4
1
|
Total No. of Tetrads
13
7
34
15
3
33
6
|
Number/Phenotype of Uniparental Progeny
13/(***) suppression of ac10-6C by SUC2
7/(0)
66% (Biparental Segregation)
11/(*)
2/(***)
32/(***)
6/(***), fast growers and SMs
|
Legend for Tables 2 and 3:
MUD2 = resistance to mxyothiazol (mitochondrial cyb gene)
SM = streptomycin resistance (chloroplast 16S gene)
ac10-6C = cannot grow photosynthetically (point mutation in rbcL)
LD1 = cannot grow under heterotrophic conditions, derived from MUD2
SUC1 = chloroplast suppressor 1 derived from LD1
SUC2 = chloroplast suppressor 2 derived from LD1
Back to chloroplast genetics essay.
|
Get your own Free Home Page